Instead, both fragments were combined directly after the PCR reaction to save time and reagents. A separate clean-up of fragments and adjustment of fragment ratios might increase success rates rein low-throughput applications. Transformations were done with 2 μL assembly product and 20 μL chemically competent Escherichia coli XL1-Blue cells. As https://josueekpty.wikiconversation.com/6075943/fascination_Über_mutagenesis